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The mission of the Genome Editing Service at The Jackson Laboratory for Genomic Medicine is to create genetically-engineered stem cell lines for use in human disease biology research and development. In recent years, genome editing techniques such as CRISPR/Cas have allowed for the creation of specific mutations in clonal cell populations, greatly advancing downstream functional readouts in experimental systems. As a model, we use human induced pluripotent stem (iPS) cells and take advantage of carefully developed protocols to create a range of desired allele types. These include knockouts, knock-ins, point mutations, and conditional alleles. After an initial consultation, our staff will design and execute an editing strategy that involves several quality control measures along the way and provide any necessary feedback during the process. We also provide consultation on allele design, gRNA selection, and editing protocols.
Justin McDonough, Ph.D.
Associate Director, Cellular Engineering
(860) 837-2402
justin.mcdonough@jax.org
| Hours | Location |
|
9:00am-5:00pm M-F |
10 Discovery Dr. Farmington, CT 06032 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Justin McDonough, Ph.D. |
Associate Director & Service Lead, Cellular Engineering
|
(860) 837-2402
|
justin.mcdonough@jax.org
|
JAX-GM (Connecticut)
|
| Mallory Ryan, M.S. |
Lab Manager
|
mallory.ryan@jax.org
|
JAX-GM (Connecticut)
|
|
| Kaitlyn Stewart |
RA
|
kaitlyn.stewart@jax.org
|
JAX-GM (Connecticut)
|
